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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 16, No. 4, 2018, pp. 247-254
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Bioline Code: rm18027
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 16, No. 4, 2018, pp. 247-254
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NFKB1 rs28362491 and pre-miRNA-146a rs2910164 SNPs on E-Cadherin expression in case of idiopathic oligospermia: A case-control study
Tunçdemir, Matem; Yenmis, Güven; Tombultürk, Kübra; Arkan, Hülya; Soydaş, Tuğba; Tek, Rasit Burak; Altıntaş, Özlem; Özkara, Hamdi & Kanıgür-Sultuybek, Gönül
Abstract
Background: A notable proportion of idiopathic male infertility cases is
accompanied by oligozoospermia; and yet, the molecular mechanisms of
fertilization problem underlying this defect are still unclear. Epithelial cadherin has
been involved in several calcium-dependent cell-to-cell adhesion events; however,
its participation in gamete interaction has also not been fully investigated.
Objective: The aim was to investigate the changes in the expression of E-cadherin,
based on the frequency of Single nucleotide polymorphisms in Nuclear Factor
Kappa-B 1 and pre-mir-146a in oligospermic men.
Materials and Methods: In this case-control study, semen and blood samples of
131 oligospermic men as the case group and 239 fertile healthy men as the control
group were analyzed. Variants single nucleotide polymorphisms rs28362491 and
rs2910164 were performed using polymerase chain reaction-restriction fragment
length polymorphism method and E-cadherin expression were determined by
immunoprecipitation studies.
Results: ins/ins genotype of rs28362491 was determined as a risk factor for
idiopathic oligospermia by 1.73 times (p=0.0218), whereas no significant
differences were found between the groups concerning pre-mir-146a rs2910164
polymorphism (p=0.2274 in case of GC genotype and p=0.9052 in case of GG
genotype). Combined genotype analysis results did not show any notable differences
between the multiple comparisons of 28362491-rs2910164 in oligospermic men and
control groups. In addition, E-cadherin expression of oligospermic men with ins/ins
genotype was significantly lower than patients with del/ins genotype (p=0.0221). E-cadherin
expression level was low in oligospermic men with respect to the control
group in presence of ins/ins genotype of NFKB1 gene.
Conclusion: These results suggest that ins allele prevents binding of surface
proteins to spermatozoa, leading to a low affinity of sperm-oocyte interaction in
oligospermic men.
Keywords
E-Cadherin; Oligospermia; Male infertility; Nuclear factor kappa B; Mir-146a.
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