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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 16, No. 9, 2018, pp. 549-556
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Bioline Code: rm18065
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 16, No. 9, 2018, pp. 549-556
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Altered expression of 3´paralogus HOX A-D clusters in endometriosis disease: A case-control study
Jahromi, Masoumeh Golestan; Aflatoonian, Reza; Afsharian, Parvaneh; Aghajanpour, Samaneh; Shahhoseini, Maryam & Aflatoonian, Abbas
Abstract
Background: Endometriosis is a prevalent gynecological disease, with limited
known etiology and more researches are required to identify its etiology. In this
manner, there is no evidence for expression and function of 3´HOX genes in 4
clusters in the limb and pelvic organs such as the uterus and its disorders (Genes in
the HOXA-D clusters are subdivided into 13 paralogous groups).
Objective: This study designed to investigate the expression profile of 5 paralogous
(1-5) in four clusters of HOX genes (A, B, C, and D) in ectopic and eutopic tissues
of women with endometriosis compared to the normal endometrium.
Materials and Methods: Samples were obtained from thirty patients (15 with and
15 without endometriosis) of reproductive age with normal menstrual cycles. The
same patient provided both eutopic and ectopic tissues and control women were
laparoscopically checked for the absence of endometriosis. The expression profile of
these HOX genes was investigated by quantitative real-time polymerase chain
reaction technique.
Results: We observed significant up-regulation of some members of HOXC and D
clusters (HOXD1, HOXD3, HOXC4 and HOXC5) in ectopic and eutopic tissues vs.
control. Also, our data showed significant down-regulation of all of HOXA and
HOXB paralogous except HOXA1 in ectopic tissues versus control.
Conclusion: Our data showed specific cluster dependent modulation of the HOX
genes expression in endometriosis (over-expression of some HOX genes in cluster C
and D and down-regulation of HOX genes in cluster A and B) in ectopic and eutopic
tissues compare to control group. Therefore, it is possible that change of expression
level of these genes in endometrium plays a role in the pathogenesis of
endometriosis.
Keywords
Endometriosis; HOX genes; Eutopic endometrium; Ectopic endometrium
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