![](/imgs/covers/rm_cover.jpg)
|
International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 18, No. 6, 2020, pp. 415-424
|
Bioline Code: rm20046
Full paper language: English
Document type: Research Article
Document available free of charge
|
|
International Journal of Reproductive BioMedicine, Vol. 18, No. 6, 2020, pp. 415-424
en |
Glutathione-dependent enzymes in the follicular fluid of the first-retrieved oocyte and their impact on oocyte and embryos in polycystic ovary syndrome: A cross-sectional study
Zal, Fatemeh; Ahmadi, Pardis; Davari, Maryam; Khademi, Fatemeh; Akbarzadeh Jahromi, Mojgan; Anvar, Zahra & Namavar Jahromi, Bahia
Abstract
Background: Oxidative stress and GSH-dependent antioxidant system plays a key role
in the pathogenesis of polycystic ovary syndrome (PCOS).
Objective: We compared glutathione peroxidase (GPx) and glutathione reductase
activities and reduced glutathione (GSH) levels in serum and follicular fluid (FF) of the
first-retrieved follicle and their impact on quality of oocyte and embryo in PCOS women
undergoing IVF.
Materials and Methods: This cross sectional study was conducted on 80 pairs of blood
samples and FF of the first-retrieved follicle from PCOS women, at the Infertility center
of Ghadir Mother and Child Hospital. The mean activity of GPx and GR, also GSH levels
in the serum and FF were compared to the quality of the first follicle and resultant
embryo.
Results: Retrieved oocytes included 53 (66.25%) MII, 17 (21.25%) MI, and 10 (12.5%)
germinal vesicles; after IVF 42 (52.50%) embryos with grade I and 11 (13.75%) with grade
II were produced. The mean values for all three antioxidants were higher in the FF
compared to serum (p < 0.001). Also all of the mean measured levels were significantly
higher in the FF of the MII oocytes compared to that of oocytes with lower grades (p
= 0.012, 0.006 and 0.012, respectively). The mean GPX activity and GSH levels were
significantly higher in the serum (p = 0.016 and 0.012, respectively) and FF (p = 0.001
for both) of the high-quality grade I embryos.
Conclusion: GSH-dependent antioxidant system functions more efficiently in the FF of
oocytes and embryos with higher quality.
Keywords
In vitro fertilization; Glutathione; Antioxidant; Oocyte; Embryo.
|
|
© Copyright [2020] - Zal et al. Alternative site location: http://www.ijrm.ir
|
|