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International Journal of Reproductive BioMedicine
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd
ISSN: 1680-6433 EISSN: 1680-6433
Vol. 18, No. 6, 2020, pp. 449-464
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Bioline Code: rm20049
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Reproductive BioMedicine, Vol. 18, No. 6, 2020, pp. 449-464
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The effect of human amniotic epithelial cell on dendritic cell differentiation of peripheral blood monocytes: An experimental study
Keshavarzi, Bahare; Tabatabaei, Meraj; Zarnani, Amir Hasan; Ramezani Tehrani, Fahime; Bozorgmehr, Mahmood & Mosaffa, Nariman
Abstract
Background: The amniotic membrane plays an important role in maintaining a healthy
pregnancy. The main population cells from amniotic membrane include human amnion
epithelial cells (hAECs) which have been shown to possess immunomodulatory
properties.
Objective: The proximity of hAECs with monocyte leads to the generation of
tollerogenic dendritic cells.
Materials and Methods: hAECs were obtained from normal pregnancy. Peripheral
blood monocytes were isolated by anti-CD14 MACS method. Co-cultures of monocytes
and hAECs were established in Transwell chambers supplemented with granulocyte-macrophage
colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) in the absence
and presence of lipopolysaccharide (LPS) to produce immature and mature DCs,
respectively. Immunophenotyping of the obtained DCs was done through flow
cytometry and the production of cytokines was measured by ELISA. Mixed leukocyte
Reaction (MLR) was also performed for the functional assessment of DCs.
Results: Immunophenotyping of [hAECs - Immature DC (iDC)] and [hAECs - iDC] + LPS
cells revealed that the expression of CD1a, CD80, CD86, CD40, HLA-DR, and CD83
markers showed no significant difference as compared with the control group (iDCs
and mDCs alone). In the [hAECs-iDCs] + LPS cells, the percentage of CD14 cells at
the ratio of 1:2.5 showed significant differences compared to the control group. The
production of IL-10 and IL-12 showed no significant difference in any of the cultures as
compared to the control groups. Also, co-cultured DCs did not inhibit proliferation of
lymphocyte.
Conclusion: Our findings show that factors secreted from cultured hAECs are unable
to generate of tollerogenic dendritic cells. To achieve a better understanding of other
mechanisms more investigations are needed.
Keywords
Amniotic membrane; Dendritic cells; Human placenta; Immunomodulation; Monocyte.
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© Copyright [2020] - Keshavarzi et al. Alternative site location: http://www.ijrm.ir
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