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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472 EISSN: 1735-1472
Vol. 11, No. 7, 2014, pp. 1919-1926
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Bioline Code: st14188
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Environment Science and Technology, Vol. 11, No. 7, 2014, pp. 1919-1926
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Bacterial consortium and axenic cultures isolated from activated sewage sludge for biodegradation of imidazolium-based ionic liquid
Markiewicz, M.; Henke, J.; Brillowska-Dąbrowska, A.; Stolte, S.; Łuczak, J. & Jungnickel, C.
Abstract
Extensive research and increasing number of
potential industrial applications made ionic liquids (ILs)
important materials in design of new, cleaner technologies.
Together with the technological applicability, the environmental
fate of these chemicals is considered and significant
efforts are being made in designing strategies to
mitigate their potential negative impacts. Many ILs are
proven to be poorly biodegradable and relatively toxic.
Bioaugmentation is known as one of the ways of enhancing
the microbial capacity to degrade xenobiotics by addition
of specialized strains. The aim of current work was to
select microbial species that could be used for bioaugmentation
in order to enhance biodegradation of ILs in the
environment. We subjected activated sewage sludge to the
selective pressure of 1-methyl-3-octylimidazolium chloride
([OMIM][Cl]) and isolated nine strains of bacteria which
were able to prevail in these conditions. Subsequently, we
utilized axenic cultures (pure cultures) of these bacteria as
well as mixed consortium to degrade this IL. In addition,
we performed growth inhibition tests and found that bacteria
were able to grow in 2 mM, but not in 20 mM
solutions of [OMIM][Cl]. The biodegradation conducted
by the isolated consortium was higher than conducted by
the activated sewage sludge when normalized by the cell
density, which indicates that the isolated strains seem
specifically suited to degrade the IL.
Keywords
Biodegradation; Growth inhibition; Ionic liquid; Axenic culture; Bioaugmentation; Ecotoxicity; Hormesis
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