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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472 EISSN: 1735-1472
Vol. 12, No. 3, 2015, pp. 1107-1122
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Bioline Code: st15102
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Environment Science and Technology, Vol. 12, No. 3, 2015, pp. 1107-1122
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Aerobic and anaerobic ammonium-oxidising bacterial enrichment from municipal solid waste
Sri Shalini, S.; Kurian, J. & Wong, J. W. C.
Abstract
Leachate from the municipal solid waste
(MSW) landfills contains high concentration of ammoniacal
nitrogen that is a major toxic pollutant that has a great
threat to environment. Among the processes available for
the removal of ammoniacal nitrogen, one process is a
combination of partial nitrification and anaerobic ammonium
oxidation (anammox) process. It requires aerobic
ammonium-oxidising bacteria (AOB) and anaerobic
ammonium-oxidising bacteria (AnAOB). This paper presents
the feasibility of enriching the AOB and AnAOB in
100-mL and 2.5-L batch reactors from fresh and mined
MSW and leachate under aerobic and anaerobic conditions
with varying feed-to-seed ratio. The AOB and AnAOB
activity was monitored by measuring the intermediates
such as hydroxylamine and hydrazine along with variations
in ammoniacal nitrogen, nitrite nitrogen, and nitrate
nitrogen concentrations in the reactor contents. The formation
of intermediates such as hydroxylamine and
hydrazine and ammoniacal nitrogen transformation data
confirmed the enrichment of AOB and AnAOB. Further,
AOB and AnAOB were validated by most probable number
test and scanning electron microscopy analysis,
respectively. DNA extraction, polymerase chain reaction
amplification, and sequencing analysis authenticated the
AnAOB as Candidatus Brocadia anammoxidans.
Keywords
Bacterial enrichment; Ammoniacal nitrogen removal; Aerobic ammonium-oxidising bacteria; Anammox bacteria; Feed-to-seed ratio; Candidatus Brocadia anammoxidans
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