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International Journal of Environment Science and Technology
Center for Environment and Energy Research and Studies (CEERS)
ISSN: 1735-1472 EISSN: 1735-1472
Vol. 12, No. 12, 2015, pp. 3837-3842
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Bioline Code: st15361
Full paper language: English
Document type: Research Article
Document available free of charge
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International Journal of Environment Science and Technology, Vol. 12, No. 12, 2015, pp. 3837-3842
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Introduction of a spectrophotometric method for simultaneous determination of nitrite and nitrate in water samples using partial least squares
Shariati-Rad, M.; Irandoust, M. & Haghighi, M.
Abstract
A simple and sensitive spectrophotometric
method for simultaneous determination of nitrite and
nitrate was introduced. The method is based on the reaction
of nitrite and nitrate with 2,4-xylenol as reagent in the
concentrated sulfuric acid. Calibration curves were linear
in the range of 0.2–20.0 and 0.5–7.0 mg L-1 for nitrite and
nitrate, respectively. Due to the severe spectral overlapping
of the reaction products of two anions with 2,4-xylenol,
partial least squares (PLS) was used for simultaneous
determination of nitrite and nitrate in the mixtures. For
calibration and validation, 25 binary mixtures of nitrite and
nitrate (17 calibration and eight external test samples) were
designed by simplex lattice design with lattice degree of
one. PLS models with Q2 values of 0.988 and 0.976 were
constructed for nitrite and nitrate, respectively. The validated
models were used to analyze real water samples.
With the recommended method, simultaneous determination
of nitrite and nitrate in water samples is possible with
satisfactory results.
Keywords
Nitrite; Nitrate; 2,4-xylenol; Partial least squares; Water samples
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