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Analysis of primary and secondary stress responses in bighead carp ( Hypophthalmichthys nobilis ) by anesthetization with 2-phenoxyethanol
Akbary, P.; Pirbeigi, A. & Jahanbakhshi, A.
Abstract
The main objective of the present study was to
shed light on the effects of 2-phenoxyethanol on possible
primary (cortisol level) and secondary (hematological
indices and glucose level) stress responses and changes in
the activity of metabolic enzymes (AST, ALT and ALP) in
bighead carp (Hypophthalmichthys nobilis). The fish were
exposed to 0.1, 0.3, 0.5, 0.7 and 0.9 ml L-1 of 2-phenoxyethanol.
2-Phenoxyethanol induction and recovery
times were 115–595 and 29.66–179.3 s, respectively. At a
concentration of 0.1 ml L-1, the anesthetic failed to induce
deep anesthesia. The lowest effective concentration of
2-phenoxyethanol was determined to be 0.7 ml L-1,
whereas 0.9 ml L-1 was found to be the most effective
one. Data showed that RBC, WBC, hemoglobin and
hematocrit values were significantly high in some treatments
compared to the control. MCV and MCH contents
decreased significantly. MCHC, ALP, AST and ALT did
not vary significantly. Plasma glucose and cortisol levels
were significantly high compared to the control (in some
doses). Moreover, Na+, K+, Ca2+, Mg2+ and Cl- did not
vary significantly. Findings suggest that blood parameters
were affected by anesthetizing bighead with 2-phenoxyethanol
at 0.9 ml L-1 to the lowest extent.
Keywords
Anesthesia; Cortisol; Fish; Glucose; Hematological
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