Background: The dried fruit of
Forsythia suspensa
(Thunb.) Vahl. (Oleaceae) are better known by their herbal name
Forsythiae Fructus
, and have a bitter taste, slightly pungent smell, and cold habit. FF has been widely used to treat symptoms associated with
the lung, heart, and small intestine. Recently, bioactive compounds isolated from hydrophobic solvent fractions of FF have been
reported to have anti-oxidant, anti-bacterial, and anti-cancer effects. Traditionally, almost all herbal medicines are water extracts, and
thus, extraction methods should be developed to optimize the practical efficacies of herbal medicines.
Materials and Methods: In this study, the anti-inflammatory effects of the ethyl acetate fraction of the methanol extract of FF (FFE)
were assessed by measuring NO and PGE
2 production by and intracellular ROS and protein levels of iNOS and COX-2 in RAW
264.7 cells.
Results: FFE inhibited COX-2 expression in LPS-stimulated RAW 264.7 cells.
Conclusion: In summary, FFE effectively reduced intracellular ROS and NO levels and inhibited PGE
2 production by downregulating
COX-2 levels.
