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African Journal of Traditional, Complementary and Alternative Medicines
African Ethnomedicines Network
ISSN: 0189-6016
Vol. 13, No. 6, 2016, pp. 135-143
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Bioline Code: tc16151
Full paper language: English
Document type: Research Article
Document available free of charge
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African Journal of Traditional, Complementary and Alternative Medicines, Vol. 13, No. 6, 2016, pp. 135-143
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PHENOLIC PROFILE OF Centaurea aegyptiaca L. GROWING IN EGYPT AND ITS CYTOTOXIC AND ANTIVIRAL ACTIVITIES
Bakr, Riham Omar; Mohamed, Shaza Abd El Halim & Ayoub, Nahla
Abstract
Background: Centaurea aegyptiaca L (Asteraceae), is one of the most attractive plants growing wildly in Sinai, and is not well
investigated for its phytochemical constituents. This study represents the first in-depth characterization of the phenolic profile of the
aerial parts of C. aegyptiaca methanolic extract utilizing liquid chromatography (LC) combined with electrospray ionization (ESI)
tandem mass spectrometry (MS/MS).
Material and Methods: Phenolic profile was researched utilizing LC-HRESI-MS-MS. Assessment of cytotoxic activity against four
human cancer cell lines (Hep-G2; hepatocellular carcinoma cells, MCF-7; breast adenocarcinoma cells, and HCT-116; colon
carcinoma and HELA; cervical carcinoma cells) was performed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium
bromide (MTT) assay. Antiviral activity was surveyed utilizing cytopathic effect inhibition assay.
Results: A total of sixty-one compounds were tentatively distinguished (twenty-one phenolic acids and their derivatives, thirty-one
flavonols and nine flavones) in the negative and positive modes. Centaurea aegyptiaca demonstrated outstanding results against
Hep-G2, MCF-7, HCT-116 and HELA cell lines with IC50 of 12.1, 30.9, 11.7 and 19.5 μg/mL respectively compared and
doxorubicin as a reference drug. Weak antiviral activity was seen against hepatitis A virus (HAV) and no impact against herpes
simplex virus type 1 (HSV 1).
Conclusion: This study provides a better understanding of the chemistry of C. aegyptiaca that announces itself as a promising
cytotoxic agent.
Keywords
Centaurea aegyptiaca; Cytotoxicity; MTT assay; flavonoid
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