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Zoological Research
Kunming Institute of Zoology, Chinese Academy of Sciences
ISSN: 2095-8137
Vol. 31, No. 2, 2010, pp. 113−121
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Bioline Code: zr10018
Full paper language: English
Document type: Research Article
Document available free of charge
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Zoological Research, Vol. 31, No. 2, 2010, pp. 113−121
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Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting
Peng, Kun-Jing; Wang, Jin-Huan; Su, Wei-Ting; Wang, Xi-Cai; Yang, Feng-Tang & Nie, Wen-Hui
Abstract
Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas
of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from
patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome
painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line
derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome
painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines.
Thirteen and twenty-four abnormal chromosomes were identified in A549 and GLC-82 cell lines, respectively. Almost
half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were
derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently
common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.
Keywords
Lung adenocarcinoma cell lines; Chromosome sorting; Chromosome painting; Cytogenetic characterization
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