Nibea albiflora
was immunized by intraperitoneal injection with either Lipopolysaccharide (LPS) or outer
membrane protein (OMP) extracted from
Vibrio vulnificus
or formalin killed
Vibrio vulnificus (FKC). The influence of
the three antigens on the immunological function of
Nibea albiflora was determined at different time points following the
injection by testing the agglutinating antibody titers of the serum, lysozyme activity of the serum, phagocytic activity of
the blood and the relative survival percentage. The results showed that the three antigens have higher immunogenicity
and antigenicity than the control group(injection with sterile saline). The agglutinating antibody titers of the immune
challenged groups increased quickly, and were highest on the day 28. The lysozyme activity and phagocytic activity were
raised significantly (P<0.01), reaching their top value on day 21, and then the index gradually reduced. The
immunological indexes of three immune groups were higher than the control group (P<0.05). The agglutinating antibody
titers of the LPS group or the OMP group were lower than the control group, but the relative survival percentage was
adverse when challenged with a
Vibrio vulnificus infection. The order of relative survival percentage was group
LPS>OMP>FKC>Control.