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African Crop Science Journal
African Crop Science Society
ISSN: 1021-9730 EISSN: 1021-9730
Vol. 3, No. 2, 1995, pp. 171-180
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Bioline Code: cs95023
Full paper language: English
Document type: Research Article
Document available free of charge
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African Crop Science Journal, Vol. 3, No. 2, 1995, pp. 171-180
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New Vistas are Opened for Sorghum Improvement by Genetic Transformation
Kononowicz, A.K.; Casas, A.M.; Tomes, D.T.; Bressan, R.A. & Hasegawa, P.M.
Abstract
Transgenic fertile sorghum plants (Sorghum bicolor L. Moench)
were obtained by microprojectile bombardment of immature embryo
and immature inflorescence explants. Regeneration from the calli
was through embryogenesis and organogenesis pathways, resulting
in both the uniformly transformed and chimeric plants. Plant
regeneration occurred on media supplemented with bialaphos.
Microprojectile bombardment resulted in plants resistant to the
herbicide and exhibited phosphinothricin acetyltransferase
activity. The presence of bar, uMa and luc genes in the T[o]
plants was confirmed by Southern blot analysis of genomic DNA.
The herbicide resistance was inherited in T[1] plants.
Keywords
Bialaphos, explants, herbicide, regeneration, Sorghum bicolor, transgenic
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fr |
Kononowicz, A.K.; Casas, A.M.; Tomes, D.T.; Bressan, R.A. & Hasegawa, P.M.
Résumé
Les plantes transgeniques fertiles de surgho (Sorghum bicolor L.
Moench ) etaient obtenues par bombardement de microprojectiles
d'embryons immatures et d'explants d'inflorescences immatures.
La regeneration a partit de cales a lieu a travers l'embryogenese
et l'organogenese qui resultent en la formation des plants
uniformement transformes et des chimeres. La regeneration des
plantes se fait sur des milieux ayant obtenu un supplement de
bialaphos. Le bombardement des microprojectiles a abouti a la
production des plants resistants aux herbicides et presentant
l'activite phosphinothricine acetyltransferase.
La presence des genes bar, uida et luc sur les plants T[o] a ete
confirmee a l'aide de l'analyse southern blot du DNA genomique.
La resistance a l'herbicide etait herite par les plants en T[1].
Mots Clés
Bialaphos, explants, herbicide, regeneration, Sorghum bicolor; transgenique
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© Copyright 1995 - African Crop Science Society
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